Composite

Part:BBa_K4010007:Design

Designed by: Yi Yang Fei   Group: iGEM21_McMaster   (2021-10-16)


Pconst + QseC + NarX & PyeaR + QseB + NarL


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 2806
    Illegal NotI site found at 2099
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1003
    Illegal BglII site found at 4418
    Illegal BglII site found at 4586
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This composite part assumes a chassis of Escherichia coli, where NsrR repressors are naturally present. If other bacteria are used, NsrR (BBa_K1682011) must be constitutively added to ensure PyeaR is being repressed when no nitric oxide is present.


Source

All parts are from the iGEM Parts Registry.

References